AustralieFrV1, Main, Exploration, bibRecord, 006477

Characterization of the cryptic Escherichia lineages: rapid identification and prevalence

Identifieur interne : 006477 ( Main/Exploration ); précédent : 006476; suivant : 006478

Characterization of the cryptic Escherichia lineages: rapid identification and prevalence

Auteurs : Olivier Clermont [France] ; David M. Gordon [Australie] ; Sylvain Brisse [France] ; Seth T. Walk [États-Unis] ; Erick Denamur [France]

Source :

Environmental Microbiology [ 1462-2912 ] ; 2011-09.

RBID : ISTEX:B0ECFEAE04FCE406F5512AFE7B5609E24BD9FAC1

English descriptors

Abstract

Strains phenotypically indistinguishable from Escherichia coli and belonging to at least five distinct cryptic lineages, named Escherichia clades I to V, that are genetically divergent from E. coli yet members of the genus have been recently found using multi‐locus sequence typing (MLST). Very few epidemiological data are available on these strains as their detection by MLST is not suitable for large‐scale studies. In this work, we developed a rapid PCR method based on aes and chuA allele‐specific amplifications that assigns a strain a cryptic lineage membership. By screening more than 3500 strains with this approach, we show that the cryptic lineages of Escherichia are unlikely to be detected in human faecal samples (2–3% frequency) and even less likely to be isolated from extra‐intestinal body sites (< 1% frequency). They are more abundant in animal faeces ranging from 3–8% in non‐human mammals to 8–28% in birds. Overall, the strains from the clade V are the most abundant and from the clade II very rare. These results suggest that members of the cryptic clades are unlikely to be of significance to human and health but may influence the use of ‘E. coli’ as an indicator of water quality.

Url:

https://api.istex.fr/document/B0ECFEAE04FCE406F5512AFE7B5609E24BD9FAC1/fulltext/pdf

DOI: 10.1111/j.1462-2920.2011.02519.x

Affiliations:

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<front><div type="abstract" xml:lang="en">Strains phenotypically indistinguishable from Escherichia coli and belonging to at least five distinct cryptic lineages, named Escherichia clades I to V, that are genetically divergent from E. coli yet members of the genus have been recently found using multi‐locus sequence typing (MLST). Very few epidemiological data are available on these strains as their detection by MLST is not suitable for large‐scale studies. In this work, we developed a rapid PCR method based on aes and chuA allele‐specific amplifications that assigns a strain a cryptic lineage membership. By screening more than 3500 strains with this approach, we show that the cryptic lineages of Escherichia are unlikely to be detected in human faecal samples (2–3% frequency) and even less likely to be isolated from extra‐intestinal body sites (< 1% frequency). They are more abundant in animal faeces ranging from 3–8% in non‐human mammals to 8–28% in birds. Overall, the strains from the clade V are the most abundant and from the clade II very rare. These results suggest that members of the cryptic clades are unlikely to be of significance to human and health but may influence the use of ‘E. coli’ as an indicator of water quality.</div>
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Characterization of the cryptic Escherichia lineages: rapid identification and prevalence

Characterization of the cryptic Escherichia lineages: rapid identification and prevalence

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English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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